Biacore 3000 Universal Approach to Ligand-Protein

Project Description Narrative:

The purchase of a Biacore3000 instrument, when combined with innovative projects focusing on neuroscience, cardiovascular disease, and cancer taking place at MCW, will allow the development of highly significant and novel studies that are directly relevant to health issues of major concern to the residents of Wisconsin, including obesity and its impact on heart disease, substance abuse, and environmental health hazards that contribute to cancers. This state-of-the-art technology allows for increased sensitivity, high through-put, and for the detection of drug-protein, hormone-protein, protein-protein, DNA-protein, carbohydrate-protein, and lipid-protein interactions that cannot be performed with other methodologies. Thus, the technology can be easily applied to many disciplines, and the instrument’s ability to interface with mass spectrometers provides direct ties with proteomics for discovery-based research, resulting in the generation of data that will be used to increase competitiveness on existing grants and for the submission of new grants.